Sprint Bioscience offer a fully equipped platform for fragment-based drug discovery comprising protein expression and purification, fragment screening, high-throughput X-ray crystallography and full expansion of fragments into a patented lead series. Contact us for further information on how we can contribute to your lead finding process.
Sprint Bioscience workflow
- Access to target protein
— Definition of target protein, screening cascade and lead compound criteria
— Development of construct and protocol for protein supply - Establishment of crystal system and biochemical assays
— Development of crystallization conditions
— Development of assays in screening funnel - Identification of fragment starting points
— Fragment screening of in-house library (500 fragments)
— Hit confirmation and evaluation by orthogonal assays
— Structure determination - Identification of lead molecule
— Optimization of fragments
— Fragment elaboration
— Design driven by iterative structure determination
— Selection of lead molecule according to criteria
— Project transfer to customer - Lead optimization support
— Crystallography and protein supply
— Chemistry synthesis protocols
— Synthesis of patent examples
